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将操纵的肿瘤相关中性粒细胞转移到肿瘤携带小鼠中,以研究其血管生成潜力在Vivo
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Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
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将操纵的肿瘤相关中性粒细胞转移到肿瘤携带小鼠中,以研究其血管生成潜力在Vivo

DOI:
10.3791/59807-v

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08:19 min

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July 20, 2019

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, , , ,
1Department of Otorhinolaryngology, University Hospital,University of Duisburg-Essen

Chapters

  • 00:04Title
  • 00:58Allogenic Tumor Mouse Model and Neutrophil Adoptive Transfer
  • 02:32Tumor Growth Evaluation
  • 03:54Tumor-Associated Neutrophil (TAN) Isolation
  • 06:04In Vitro FK866-TAN Inhibition
  • 06:44Results: Representative Analyses of FK866 Treatment on Neutrophil Angiogenic Properties and Tumor Growth
  • 07:32Conclusion

Summary

자동 번역

자동 번역

在这里,我们展示了抗血管性肿瘤相关中性粒细胞转移到肿瘤小鼠后的治疗潜力。该协议可用于操纵体内的嗜中性粒细胞活性,并随后评估其在体内的肿瘤发展功能。它是研究潜在的中微粒细胞免疫疗法的适当模型。

Tags

Tumor-associated NeutrophilsAngiogenic PotentialNAMPT Inhibitor FK866B16F10 MelanomaAdoptive TransferTumor-bearing MiceTumor GrowthHistological AnalysisCryosectioning

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