Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

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생화학

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JoVE 신문 생화학

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

DOI:

10.3791/59820-v

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11:23 min

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June 30, 2019

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Béatrice Vallée, Michel Doudeau, Fabienne Godin, Hélène Bénédetti

¹Centre de Biophysique Moléculaire, CNRS, UPR 4301,University of Orléans and INSERM

Chapters

00:04Title
01:24Transient Transfections
03:21Lysis
05:17Immunoprecipitation
06:59Coimmunoprecipitation Analyses
08:33Results: Synthesized LIMK2-1 Protein, Its Interaction with Kinase ROCK, its Kinase Activity
10:10Conclusion

Summary

자동 번역

We characterized a new kinase protein using robust biochemical approaches: Western Blot analysis with a dedicated specific antibody on different cell lines and tissues, interactions by coimmunoprecipitation experiments, kinase activity detected by Western Blot using a phospho-specific antibody and by γ[³²P] ATP labeling.

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

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Chapters

Summary

자동 번역

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