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Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
JoVE 신문
생물학
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JoVE 신문 생물학
Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

DOI:
10.3791/59822-v

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12:04 min

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June 24, 2019

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, , ,
1Program in Molecular and Computational Biology,University of Southern California, 2Leonard Davis School of Gerontology,University of Southern California

Chapters

  • 00:04Title
  • 01:06Fission Yeast Culture and Sample Preparation
  • 07:12Live-cell Imaging
  • 08:09Image Analysis
  • 10:05Results: Cell Selection, Mitotic and Meiotic Nuclear Dynamics
  • 11:22Conclusion

Summary

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자동 번역

Here, we present live-cell imaging which is a non-toxic microscopy method that allows researchers to study protein behavior and nuclear dynamics in living fission yeast cells during mitosis and meiosis.

Tags

Live Cell MicroscopyFission YeastNuclear DynamicsMitosisMeiosisFluorescence ImagingCell PreparationAgarose PadMicroscope Slide SetupTime-lapse Imaging

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