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In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells
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In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells

In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells

DOI:
10.3791/59826-v

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09:29 min

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July 03, 2019

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, ,
1Division of Cardiology, Department Medicine,Johns Hopkins School of Medicine

Chapters

  • 00:04Title
  • 00:44Mouse Embryonic Stem Cell (mESC) Maintenance
  • 01:45Cardiac Progenitor Cell (CPC) Generation
  • 03:13Heart Field-specific CPC Isolation by Fluorescent Protein Reporter Expression
  • 04:22Heart Field-specific CPC Isolation by Cell Surface Protein Marker Expression
  • 05:26Heart Field-Specific CPC Analysis
  • 07:18Results: Representative CPC Differentiation Characterization
  • 08:57Conclusion

Summary

자동 번역

자동 번역

The purpose of this method is to generate heart field-specific cardiac progenitor cells in vitro in order to study the progenitor cell specification and functional properties, and to generate chamber specific cardiac cells for heart disease modelling.

Tags

Keywords: Heart Field-specific Cardiac Progenitor CellsMouse Embryonic Stem CellsCongenital Heart DiseaseCardiogenesisCardiac SpheroidsActivin ABone Morphogenetic Protein 4SFD Medium2i MediumIn Vitro Generation

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