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人脐带血衍生CD34的泛骨髓分化^–造血干细胞和祖细胞

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Pan-myeloid Differentiation of Human Cord Blood Derived CD34⁺ Hematopoietic Stem and Progenitor Cells

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人脐带血衍生CD34的泛骨髓分化^–造血干细胞和祖细胞

DOI:

10.3791/59836-v

•

10:25 min

•

August 09, 2019

•

Aditi Bapat, Nakia Keita, Shalini Sharma

¹Department of Basic Medical Sciences, College of Medicine-Phoenix,University of Arizona

Chapters

00:04Title
00:46Human Umbilical Cord Blood Mononuclear Cell (MNC) Isolation
02:45CD34^+ Cell Isolation from MNCs
04:55Flow Cytometric Analysis of Hematopoietic Stem and Progenitor Cell (HSPC) Populations
05:59CD34^+ HSPC Myeloid Differentiation
08:06Results: Representative Characterization of Freshly-isolated and Differentiated HSPCs
09:47Conclusion

Summary

자동 번역

在这里,我们提出了免疫表征表表征和细胞因子诱导分化脐带血衍生CD34^-造血干细胞和祖细胞到四个骨髓系的方案。该协议的应用包括研究骨髓疾病突变或小分子对CD34+细胞骨髓分化的影响。

Tags

Myeloid Differentiation CD34+ Hematopoietic Stem And Progenitor Cells Umbilical Cord Blood Mononuclear Cell Isolation Density Gradient Centrifugation CD34 Positive Cell Isolation

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