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1Department of Molecular Medicine,Sapienza University, 2Department of Internal Medicine – DMISM,Sapienza University, 3Center for Life Nano Science@Sapienza,Istituto Italiano di Tecnologia, 4Pasteur Institute Italy-Fondazione Cenci Bolognetti, 5INSERM U1052-Cancer Research Center of Lyon, 6Department of Hepatology,Croix Rousse Hospital, Hospices Civils de Lyon
Chapters
- 00:04Title
- 00:32Thawing, Amplification, and Cryopreservation of HepaRG Cells
- 02:20Differentiation of HepaRG cells (Day 0 21) and Induction of Vesicular Steatosis (Day 21 26)
- 04:25Evaluation of Lipid Overloading and Steatosis Induction: Oil Red O Staining
- 06:04Evaluation of Lipid Overloading and Steatosis Induction: Bodipy Staining
- 07:02Results: Induction and Quantification of Vesicular Steatosis in HepaRG Cells
- 08:34Conclusion
I denne undersøgelse beskriver vi en detaljeret protokol for at inducere levervesikulær steatose i differentierede heparg-celler med fedtsyresalt natriumoleat og anvende metoder til påvisning og kvantificering af lipid-akkumulering, herunder sammenhængende anti-Stokes Raman spredning (biler) mikroskopi, cytofluorimetriske analyse, olie rød O farvning, og qPCR.
