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1Department of Biochemistry and Immunology, Ribeirão Preto Medical School,University of São Paulo, 2Department of Physiology, Ribeirão Preto Medical School,University of São Paulo, 3Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School,University of São Paulo, 4Department of Anatomy, Institute of Biosciences,São Paulo State University, 5Clinical Hospital at the Ribeirão Preto Medical School,University of São Paulo
Chapters
- 00:04Title
- 01:14Setting Up the Slicing Apparatus
- 02:14Sample Collection
- 02:45Slicing
- 04:14Culture
- 05:18Morphological Evaluation
- 06:39Results: Analysis of the Short-Term Free-Floating Slice Cultures
- 08:12Conclusion
A protocol to prepare free-floating slice cultures from adult human brain is presented. The protocol is a variation of the widely used slice culture method using membrane inserts. It is simple, cost-effective, and recommended for running short-term assays aimed to unravel mechanisms of neurodegeneration behind age-associated brain diseases.
