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In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts In Vivo
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:06Title
- 00:44Preparation of Embryos for Implantation
- 01:31Perivitelline Injection of Mammalian Cancer Cells into 2 dpf (Day Post Fertilization) Embryos
- 03:55Live Imaging and Quantitation of the Angiogenic Response to the Zebrafish Xenograft
- 08:34Results: Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
- 10:37Conclusion
Le but de cette méthode est de générer un modèle in vivo de l'angiogenèse tumorale en xénogreffer les cellules tumorales mammifères dans un embryon de poisson zèbre qui a des vaisseaux sanguins fluorescents étiquetés. En imagerie du xénogreffe et des vaisseaux associés, une mesure quantitative de la réponse angiogénique peut être obtenue.
