JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Laboratoire de Biochimie et Biologie Moléculaire, Groupe Hospitalier Sud,Hospices Civils de Lyon, 2Circulating Cancer (CIRCAN) Program,Hospices Civils de Lyon Cancer Institute, 3University of Lyon, Claude Bernard University,Cancer Research Center of Lyon, 4Biolidics Limited, 5Institut de Pathologie Multisites des HCL-Site Sud,Hospices Civils de Lyon, 6Acute Respiratory Disease and Thoracic Oncology Department, Lyon Sud Hospital,Hospices Civils de Lyon Cancer Institute, 7EMR-3738 Therapeutic Targeting in Oncology, Lyon Sud Medical Faculty,Lyon 1 University
Chapters
- 00:04Title
- 01:11Pre-Analytical Circulating Tumor Cells (CTC) Enrichment from Patient Whole Blood Samples
- 03:10Spiral Microfluidic Device CTC Enrichment
- 04:20Immunofluorescence (IF) Staining
- 06:32IF Image Acquisition and Analysis
- 08:07Results: Representative CTC Characterization and Enumeration
- 09:56Conclusion
La caractérisation des cellules tumorales circulantes (CTC) est un sujet populaire dans la recherche translationnelle. Ce protocole décrit un test semi-automatique d'immunofluorescence (IF) pour la caractérisation de PD-L1 et l'énumération des CTC dans les échantillons patients de cancer de poumon de non-petite cellule (NSCLC).
