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用于病毒感染活成像和定量的路西酶-荧光报告流感病毒
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection
DOI:

05:21 min

August 14, 2019

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Chapters

  • 00:04Title
  • 01:04Mouse Infection
  • 01:32Bioluminescence Monitoring of Mice Infected with BIRFLU
  • 02:32Fluorescence Analysis in Mice Infected with BIRFLU
  • 03:32Results: BIRFLU Characterization and Tracking
  • 04:58Conclusion

Summary

자동 번역

甲型流感病毒(IAv)是传染性呼吸道病原体,每年流行和偶尔大流行。在这里,我们描述了一种使用新型重组荧光素酶和荧光表达双报告IAV(BIRFLU)跟踪体内病毒感染的协议。这种方法为研究人员提供了一个极好的工具来研究IAV在体内。

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