Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

Michael Seager; Dara  L. Aisner; Kurtis  D. Davies

doi:10.3791/59895

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Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

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Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

DOI:

10.3791/59895-v

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09:49 min

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July 05, 2019

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Michael Seager, Dara L. Aisner, Kurtis D. Davies

¹Department of Pathology,University of Colorado – Anschutz Medical Campus

Chapters

00:04Title
00:49Random Priming and First Strand cDNA Synthesis
01:55Ligation Step 2 and Bead Purification
04:25First PCR and Bead Purification
05:48Library Quantitation and Sequencing
07:41Assay Data Interpretation
08:39Results: Sample Summary and Fusion Calls
09:27Conclusion

Summary

자동 번역

This article details the use of an anchored multiplex polymerase chain reaction-based library preparation kit followed by next-generation sequencing to assess for oncogenic gene fusions in clinical solid tumor samples. Both wet-bench and data analysis steps are described.

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Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

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