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Chapters
- 00:04Title
- 00:55Deparaffinization and Rehydration
- 02:24Slide Staining
- 05:05DAPI Application
- 05:55Imaging of Monoplexes and Multiplex Preparation
- 07:18Results: Monoplex and Multiplex Optimization
- 08:39Conclusion
Multiplex fluorescent immunohistochemistry is an emerging technology that enables the visualization of multiple cell types within intact formalin-fixed, paraffin embedded (FFPE) tissue. Presented are guidelines for ensuring a successful 7-color multiplex with instructions for optimizing antibodies and reagents, preparing slides, design and tips for avoiding common problems.
