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零售食品样品中丁酸二氮的浓缩和检测

JoVE 신문
환경과학

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JoVE 신문 환경과학

Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples

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零售食品样品中丁酸二氮的浓缩和检测

DOI:

10.3791/59931-v

•

08:00 min

•

October 18, 2019

•

Zuha Anwar*¹, Samantha B. Regan*¹, Jennifer Linden

¹Brain and Mind Institute,Weill Cornell Medical College

Chapters

00:04Title
00:42Media Preparation and Sample Collection
02:48TSC Sandwich Plating
04:05Sub-culturing of Sulfite-Reducing Colonies
05:08DNA Extraction and PCR Genotyping
07:29Conclusion

Summary

자동 번역

该协议的目的是检测当地购买食品中不同的梭菌毒素毒素，特别是产生B型和D型的乙酸毒素，而无需使用厌氧室。

Tags

Clostridium Perfringens Toxinotypes Enrichment Detection Retail Food Samples Epsilon Toxin Modified Rapid Perfringens Medium (RPM)D-cycloserine Tryptose Sulfite Cycloserine (TSC) Agar Anaerobic Culture Spore Selection

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