This content is Free Access.
1MRC Centre for Regenerative Medicine,University of Edinburgh, 2UCL Great Ormond Street Institute of Child Health,University College London, 3Division of Biosciences, Department of Life Sciences, College of Health and Life Sciences,Brunel University London, 4School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute,Trinity College Dublin
Chapters
- 00:04Title
- 00:31Preparation of Agarose Microplate Molds
- 01:12Seeding Human Pluripotent Stem Cells into Agarose Microwell Plates
- 03:07Differentiating hPSCs to 3D Hepatospheres
- 05:28Results: Hepatosphere Structure and Function
- 06:16Conclusion
Denne protokollen beskriver en tilnærming til å produsere hepatospheres fra menneskelige Pluripotent stamceller ved hjelp av et definert kultur system og celle selv montering. Denne protokollen er reproduserbar i en rekke cellelinjer, kostnadseffektive og tillater produksjon av stabile menneskelige hepatospheres for biomedisinsk anvendelse.
