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Chapters
- 00:04Title
- 01:04Primer Design
- 01:56PCR and DNA Digestion
- 03:23Isolation of the Finished Plasmid
- 04:15Transfection and Protein Expression
- 05:56Monitoring Receptor-β-arrestin 1 and 2 Interactions in HEK293 Cells
- 07:07Results: GLP-1r-β-arrestin 1 and 2 Orientation Screening and Interactions
- 07:44Conclusion
GPCR-β-arrestin interactions are an emerging field in GPCR drug discovery. Accurate, precise and easy to set up methods are necessary to monitor such interactions in living systems. We show a structural complementation assay to monitor GPCR-β-arrestin interactions in real time living cells, and it can be extended to any GPCR.
