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Chapters
- 00:04Title
- 00:41Glass Coverslip Activation
- 01:15Fluorescent Microbead Deposition and Hydrogel Casting
- 02:38Hydrogel Activation and Extracellular Matrix Coating
- 03:38Cell Plating and Micropipette Preparation
- 05:51Micromanipulator Calibration and Durotaxis Assay
- 07:01Results: Representative Durotactic Stimulation Responses and Protein Expression Analyses
- 07:55Conclusion
Mechanical forces are important for controlling cell migration. This protocol demonstrates the use of elastic hydrogels that can be deformed using a glass micropipette and a micromanipulator to stimulate cells with a local stiffness gradient to elicit changes in cell structure and migration.
