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Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
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JoVE 신문 면역학 및 감염병학
Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

DOI:
10.3791/60015-v

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07:25 min

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December 17, 2019

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,
1Department of Medicine,Washington University School of Medicine, 2Department of Immunology and Pathology,Washington University School of Medicine, 3Department of Developmental Biology,Washington University School of Medicine

Chapters

  • 00:05Title
  • 01:14Preparation of Human Cardiac Tissue Specimens
  • 01:41Preparation of the Mouse Heart
  • 02:11Preparation of Single Cell Suspension
  • 04:50Antibody Staining
  • 05:33Results: Flow Cytometry Gating Scheme
  • 06:22Conclusion

Summary

자동 번역

자동 번역

Presented here is a protocol to isolate various subsets of macrophages and other non-immune cells from human and mouse myocardium by preparing a single cell suspension through enzymatic digestion. Gating schemes for flow cytometry based identification and characterization of isolated macrophages are also presented.

Tags

Keywords: Macrophage SubsetsStromal CellsCardiac InflammationCardiac RepairImmune Cell DiversitySingle Cell SuspensionViable Immune CellsNonimmune Cell PopulationsMurine HeartCollagenase IDNase IHyaluronidaseDMEMDigestion

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