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色质域新形成研究方法

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A Method to Study de novo Formation of Chromatin Domains

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色质域新形成研究方法

DOI:

10.3791/60039-v

•

00:07 min

•

August 23, 2019

•

Ozgur Oksuz^2,3, Danny Reinberg²

¹Howard Hughes Medical Institute,New York University School of Medicine, ²Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, ³Whitehead Institute for Biomedical Research

Chapters

00:04Title
00:37Generation of Clonal EED Knockout Mouse Embryonic Stem Cells
02:51Engineering Cre-ERT2 Based Inducible EED Expression
04:11Following Nucleation and Spreading of PRC2 Activity on Chromatin
05:10Results: Validations and Representative Applications of Inducible EED Rescue Systems
06:43Conclusion

Summary

자동 번역

该方法旨在跟踪细胞系中PRC2介导染色质域的形成，该方法可适用于许多其他系统。

Tags

Keywords: Chromatin Domains PRC2 CRISPR EED Mouse Embryonic Stem Cells Genotyping Donor Template DNA

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