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Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery
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Chapters
- 00:05Title
- 01:48Preparation of Bacteria for In Vivo Infection Studies
- 03:09Intra-cisternal Injection of Mice
- 05:07Evaluation of Colony Forming Units (CFU) Counts in Peripheral Organs
- 06:39Preparation of Brain Tissues for CFU Count
- 08:05Results: Survival of Mice Following Intracisternal Injection of Wild-type and Mutant N. meningitidis
- 09:28Conclusion
Here, we describe a method to induce meningococcal meningitis through an intracisternal route of infection in adult mice. We present a step by step protocol of meningococcal infection from the preparation of inoculum to the intracisternal infection; then record the animal survival and evaluate the bacterial loads in murine tissues.
