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면역학 및 감염병학

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人类S100A12及其用于免疫细胞刺激的Ion诱导寡聚物的纯化

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

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人类S100A12及其用于免疫细胞刺激的Ion诱导寡聚物的纯化

DOI:

10.3791/60065-v

•

12:55 min

•

September 29, 2019

•

Sabrina Fuehner, Dirk Foell, Christoph Kessel

¹Department of Pediatric Rheumatology and Immunology,University Children’s Hospital

Chapters

00:05Title
01:02Anion-exchange Chromatography
03:53Calcium-dependent Hydrophobic-interaction Chromatography (HIC)
05:26Removal of Endotoxin and Oligomer Separation
07:20Functional Testing on Monocytes
10:22Results: Oligomers Separation
11:59Conclusion

Summary

자동 번역

该协议描述了一种用于重组无标记标记的钙结合蛋白S100A12及其用于人类单核细胞刺激测定的由水因诱导的寡聚物的纯化方法。

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