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Mechanistisch inzicht in de ontwikkeling van TNBS-gemedieerde intestinale fibrose en evaluatie van de remmende effecten van Rapamycine
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin
DOI:

10:21 min

September 12, 2019

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Chapters

  • 00:04Title
  • 00:52Induction of TNBS Fibrosis and Rapamycin Treatment in Mice
  • 01:58Isolation of Intestinal Lamina Propria and Purification of Cx3Cr1+ Mononuclear Phagocytes
  • 04:01Purification of Mononuclear Phagocytes from the Collected Cells Purification: Magnetic Purification
  • 04:51Purification of Mononuclear Phagocytes from the Collected Cells Purification: FACS Sorting
  • 05:39Flow Cytometry Analysis
  • 07:39Results: Analysis of TNBS-Mediated Intestinal Fibrosis
  • 09:27Conclusion

Summary

자동 번역

In deze studie beschrijven we een gedetailleerde procedure van TNBS-gemedieerde intestinale fibrose, die vergelijkbare pathofysiologie vertoont voor de fibrose van Crohn. We bespreken deze aanpak ook in het licht van rapamycine vergemakkelijkt remmende effecten op intestinale fibrose.

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