Journal
/
생체공학
/
Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

DOI:
10.3791/60103-v

•

00:10 min

•

August 21, 2019

•

, , ,
1U.S. Military HIV Research Program,Walter Reed Army Institute of Research, 2Henry M. Jackson Foundation for the Advancement of Military Medicine, 3Alpha-O Peptides AG

Chapters

  • 00:04Title
  • 00:43Lysis of E. Coli BL21(DE3) Containing Six-Helix Bundle Self-assembling Protein Nanoparticles
  • 01:24Protein Purification
  • 05:14Purity Assessment and Protein Identification
  • 06:28SHB-SAPN Refolding
  • 07:46Dynamic Light Scattering (DLS)
  • 08:30Results: Representative SHB-SAPN Characterization
  • 10:07Conclusion

Summary

자동 번역

자동 번역

A detailed method is provided here describing the purification, refolding, and characterization of self-assembling protein nanoparticles (SAPNs) for use in vaccine development.

Tags

Keywords: E. ColiSelf-assembling Protein NanoparticlesSAPNVaccinesTrimeric Epitope PresentationProtein PurificationProtein RefoldingProtein CharacterizationSix-helix BundleFPLCAffinity ChromatographyHisTrap HP ColumnProtein ExpressionLarge-scale ProductionClinical Trials

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/60103?article_type=v

Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
check_url/kr/60103?article_type=v

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
check_url/kr/60103?article_type=v

Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel
check_url/kr/60103?article_type=v

Analysis of Targeted Viral Protein Nanoparticles Delivered to HER2+ Tumors
check_url/kr/60103?article_type=v

PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS
check_url/kr/60103?article_type=v

Harmonic Nanoparticles for Regenerative Research
check_url/kr/60103?article_type=v

Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles
check_url/kr/60103?article_type=v

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
check_url/kr/60103?article_type=v

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods
check_url/kr/60103?article_type=v

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Read Article