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3D球形模型作为癌症相关纤维细胞分化和入侵体内研究的更多生理系统

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A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

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3D球形模型作为癌症相关纤维细胞分化和入侵体内研究的更多生理系统

DOI:

10.3791/60122-v

•

06:27 min

•

August 08, 2019

•

Ana C. Martins Cavaco², Johannes A. Eble

¹Institute of Physiological Chemistry and Pathobiochemistry,University of Münster, ²Instituto de Medicina Molecular, Lisbon

Chapters

00:04Title
00:413D Spheroids as an In Vitro Model for Fibroblast/CAF Differentiation Using Different TGF-β1 Inhibiting Compounds of Cell-matrix Interaction
02:02Immunofluorescent Staining of Spheroids
04:44Results: A 3D Spheroid Model for Cancer-Associated Fibroblasts Differentiation and Invasion
05:37Conclusion

Summary

자동 번역

该协议的目标是建立一个3D体外模型,以研究肿瘤状环境中癌症相关成纤维细胞(CAFs)的分化,可在免疫荧光、转录等不同分析系统中解决。分析和生命细胞成像。

Tags

Keywords: 3D Spheroid Model Cancer-associated Fibroblasts Fibroblast Differentiation Tumor-like Environment Cell Matrix Interaction Methylcellulose Spheroid Formation TGF-beta Cell Culture In Vitro Study

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