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Measuring Diurnal Rhythms in Autophagic and Proteasomal Flux
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Measuring Diurnal Rhythms in Autophagic and Proteasomal Flux

Measuring Diurnal Rhythms in Autophagic and Proteasomal Flux

DOI:
10.3791/60133-v

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09:18 min

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September 17, 2019

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, ,
1Division of Pulmonary and Critical Care Medicine,Washington University School of Medicine

Chapters

  • 00:04Title
  • 00:40Protease Inhibitor Administration
  • 01:31Tissue Acquisition and Biochemical Fractionation
  • 05:10Western Blotting
  • 06:48Data Analysis
  • 08:06Results: Representative Experimental Flux Results
  • 08:36Conclusion

Summary

자동 번역

자동 번역

We describe our protocol for measuring biological rhythms in protein catabolism via autophagy and the proteasome in mouse liver.

Tags

AutophagyProteasomal FluxCircadian RhythmsProtein CatabolismLeupeptinBortezomibHomogenization BufferDounce HomogenizerPost-nuclear SupernatantProtein Concentration

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