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单细胞微吸气作为荧光激活细胞分类的替代策略，用于巨型病毒混合物分离

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Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation

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单细胞微吸气作为荧光激活细胞分类的替代策略，用于巨型病毒混合物分离

DOI:

10.3791/60148-v

•

09:50 min

•

October 27, 2019

•

Dehia Sahmi-Bounsiar, Paulo Victor de Miranda Boratto, Graziele Pereira Oliveira, Jacques Yaacoub Bou Khalil, Bernard La Scola², Julien Andreani

¹Institut Hospitalo-Universitaire (IHU) – Méditerranée Infection, ²Microbes, Evolution, Phylogeny and Infection (MEΦI), Aix-Marseille Université UM63, Institut de Recherche pour le Développement IRD 198,Assistance Publique – Hôpitaux de Marseille (AP-HM)

Chapters

00:04Title
01:37Amoeba Culture
02:44Propagation of Stock Virus in Amoebae
03:33End-point Dilution and Single Cell Micro-aspiration
06:09PCR Screening
07:06Results: Optimization of the Isolation of Novel Viruses
08:17Conclusion

Summary

자동 번역

在这里，我们描述了一个单细胞微吸入方法分离受感染的阿米巴。为了分离受福斯托病毒和未知巨病毒感染的Vermamoeba病毒的病毒亚群，我们开发了下面详述的规程，并展示了其分离两种低丰度的新型巨病毒的能力。

Tags

Single Cell Micro-aspiration Fluorescence-activated Cell Sorting Giant Virus Virus Separation Virus Isolation Turnover Virus Clandisinal Virus Uzibati Virus Faustovirus Indirect Strategy Infected Host Molecular Biology Electronic Microscopy Vermamoeba Vermiformis PYG Medium Starvation Medium Multiplicity Of Infection

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