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Chapters
- 00:04Title
- 01:37Amoeba Culture
- 02:44Propagation of Stock Virus in Amoebae
- 03:33End-point Dilution and Single Cell Micro-aspiration
- 06:09PCR Screening
- 07:06Results: Optimization of the Isolation of Novel Viruses
- 08:17Conclusion
Her beskriver vi en enkelt celle mikro-aspiration metode til adskillelse af inficerede amoebae. For at adskille virale subpopulationer i Vermamoeba vermiformis inficeret af Faustovira og ukendte gigantiske vira, vi udviklede protokollen nedenfor og viste sin evne til at adskille to lav-overflod roman Giant vira.
Tags
Keywords: Single Cell Micro-aspirationFluorescence-activated Cell SortingGiant VirusVirus SeparationVirus IsolationTurnover VirusClandisinal VirusUzibati VirusFaustovirusIndirect StrategyInfected HostMolecular BiologyElectronic MicroscopyVermamoeba VermiformisPYG MediumStarvation MediumMultiplicity Of Infection
