/

면역학 및 감염병학

/

从人类母乳中分离白细胞，用于HIV靶点抗体依赖性细胞噬菌体测定

JoVE 신문
면역학 및 감염병학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 면역학 및 감염병학

Isolation of Leukocytes from Human Breast Milk for Use in an Antibody-dependent Cellular Phagocytosis Assay of HIV Targets

Please note that all translations are automatically generated. Click here for the English version.

从人类母乳中分离白细胞，用于HIV靶点抗体依赖性细胞噬菌体测定

DOI:

10.3791/60149-v

•

08:12 min

•

September 06, 2019

•

Rebecca L.R. Powell, Alisa Fox

¹Division of Infectious Diseases,Icahn School of Medicine at Mount Sinai

Chapters

00:04Title
00:44Target Microsphere Preparation
02:20Antibody-Dependent Cellular Phagocytosis (ADCP) Assay Plate Preparation
02:53Breast Milk Cell Isolation
03:48ADCP Assay
05:11Flow Cytometric Analysis
06:21Results: Representative Flow Cytometric and ADCP Analyses of HIV Targets
07:42Conclusion

Summary

자동 번역

母乳传播人体免疫缺陷病毒（HIV），尽管只有15%的感染艾滋病毒的母亲母乳喂养的婴儿被感染。母乳喂养的婴儿每天摄入+10⁵⁺¹⁰⁸母性白细胞，尽管这些细胞研究不足。在这里，我们描述了乳腺癌白细胞的分离，并分析了它们的噬菌体能力。

Tags

Artificial Intelligence AI Writing Assistants Content Generation Copywriting Time-saving Efficiency Writer’s Block Blog Posts Social Media Posts Articles Text Generation

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

V3的HIV - 1取向的基因型推理使用人口为基础的测序

脑浸润白细胞的分离

生物能量和氧化突发：协议用于人类白细胞和血小板的隔离与评价

从人类母胎界面分离白细胞

从小鼠组织在母胎界面分离白细胞

从HIV-1阳性者的血浆分离外来体的

从小鼠皮肤使用酶消化和梯度分离提取白细胞浸润的

单核细胞单层试验中用于评估Fcγ受体介导的吞噬作用

细胞凋亡细胞的吞噬作用<em>果蝇</em>胚胎

巨噬细胞中含有吞噬的鼠伤寒沙门氏菌的分离

Read Article