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用于向小鼠眼睛输送生物制剂的封装细胞技术

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JoVE 신문 생체공학

Encapsulated Cell Technology for the Delivery of Biologics to the Mouse Eye

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用于向小鼠眼睛输送生物制剂的封装细胞技术

DOI:

10.3791/60162-v

•

06:10 min

•

March 30, 2020

•

Marwa Belhaj, Balasubramaniam Annamalai, Nathaniel Parsons, Andrew Shuler, Jay Potts, Bärbel Rohrer^3,4

¹Department of Cell Biology,University of South Carolina, ²Department of Ophthalmology, Division of Research,Medical University of South Carolina, ³Department of Neuroscience, Division of Research,Medical University of South Carolina, ⁴Division of Research,Ralph H. Johnson VA Medical Center

Chapters

00:04Introduction
00:51Cell Culture and Encapsulation
02:49Confirmation of Cell Viability and Capsule Size
04:56Results: Cell Encapsulation and Delivery into the Eye
05:32Conclusion

Summary

자동 번역

此处介绍的是一种协议，用于将藻酸盐作为聚合物在永生细胞的微封装中，用于长期向啮齿动物的眼睛提供生物制剂。

Tags

Keywords: Encapsulated Cell Technology Biologics Delivery Mouse Eye Alginate Microcapsules Retinal Epithelial Cells Cell Encapsulation Sustained Drug Delivery LIVE/DEAD Assay Cell Viability

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