JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Title
- 00:55Porcine Eye Preparation
- 01:35Dissection and Incubation
- 03:43Microscopy and Endothelium Examination
- 05:11Corneal Endothelium Image Processing
- 06:15Corneal Endothelial Cell Density and Morphological Parameter Analysis
- 06:49Results: Representative Corneal Button Culture Analyses
- 08:12Conclusion
Here, a step-by-step protocol for the preparation and cultivation of porcine split corneal buttons is presented. As this organo-typically cultivated organ culture model shows cell death rates within 15 days, comparable to human donor corneas, it represents the first model allowing long-term cultivation of non-human corneas without adding toxic dextran.
