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Chapters
- 00:05Title
- 01:05Spawning Agent Administration
- 02:08B. gauderio Embryo Fertilization
- 04:25Single Cell Microinjection
- 06:19Results: Representative CRISPR/Cas9 Induced Mutations in B. gauderio and B. brachyistius
- 07:17Conclusion
Here, a protocol is presented to produce and rear CRISPR/Cas9 genome knockout electric fish. Outlined in detail are the required molecular biology, breeding, and husbandry requirements for both a gymnotiform and a mormyrid, and injection techniques to produce Cas9-induced indel F0 larvae.
