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Chapters
- 00:04Title
- 00:36Live Cell Imaging Preparation
- 02:06Microscope Setup
- 03:55Time-Lapse Image Analysis
- 05:09Results: Representative Time-Lapse Imaging of Mitotic Fidelity
- 06:32Conclusion
Here we present a protocol to assess the dynamics of spindle formation and mitotic progression. Our application of time-lapse imaging enables the user to identify cells at various stages of mitosis, track and identify mitotic defects, and analyze spindle dynamics and mitotic cell fate upon exposure to anti-mitotic drugs.
