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Live Cell Imaging om de dynamiek van de metafase timing en het lot van de cel na de mitotische spindel perturbations te beoordelen
JoVE 신문
생물학
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JoVE 신문 생물학
Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
DOI:

07:14 min

September 20, 2019

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Chapters

  • 00:04Title
  • 00:36Live Cell Imaging Preparation
  • 02:06Microscope Setup
  • 03:55Time-Lapse Image Analysis
  • 05:09Results: Representative Time-Lapse Imaging of Mitotic Fidelity
  • 06:32Conclusion

Summary

자동 번역

Hier presenteren we een protocol om de dynamiek van spindel vorming en mitotische progressie te beoordelen. Onze toepassing van time-lapse Imaging stelt de gebruiker in staat om cellen te identificeren in verschillende stadia van mitose, sporen en identificeren mitotische defecten, en analyseren spil dynamiek en mitotische cel lot bij blootstelling aan anti-mitotische drugs.

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