Journal
/
암 연구학
/
Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy
JoVE 신문
암 연구학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 암 연구학
Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy

Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy

DOI:
10.3791/60273-v

•

09:17 min

•

September 16, 2019

•

, ,
1School of Applied Sciences,University of Huddersfield, 2Laboratory of Radiotherapy and Experimental Radiation Oncology, Humbertus Wald Tumorzentrum,University Cancer Center Hamburg, University Medical Center Hamburg-Eppendorf

Chapters

  • 00:04Title
  • 01:01Generation of Cell Spheroids
  • 02:15Collagen Invasion Assay
  • 04:15Preparation of Collagen Embedded Spheroids and Migratory Cells for Confocal Microscopy
  • 07:13Results: Cell Migration and Invasion
  • 08:20Conclusion

Summary

자동 번역

자동 번역

The effects of migrastatic inhibitors on glioma cancer cell migration in three-dimensional (3D) invasion assays using a histone deacetylase (HDAC) inhibitor are characterized by high-resolution confocal microscopy.

Tags

Keywords: 3D Tumor SpheroidMigrastatic InhibitorsConfocal Microscopy암 연구학Cytotoxic DrugsCell MigrationCell Proliferation3D Cell CultureGliomaCollagenCell Invasion

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/60273?article_type=v

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics
check_url/kr/60273?article_type=v

Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window
check_url/kr/60273?article_type=v

Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy
check_url/kr/60273?article_type=v

Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH
check_url/kr/60273?article_type=v

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model
check_url/kr/60273?article_type=v

A 3D Organotypic Melanoma Spheroid Skin Model
check_url/kr/60273?article_type=v

Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion
check_url/kr/60273?article_type=v

Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models
check_url/kr/60273?article_type=v

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
check_url/kr/60273?article_type=v

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

Read Article