Generation of In-Frame Gene Deletion Mutants in <em>Pseudomonas aeruginosa</em> and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Meagan  E. Valentine; Brandon  D. Kirby; Hongwei D. Yu

doi:10.3791/60297

Journal

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면역학 및 감염병학

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在伪单体中生成帧内基因删除突变体，并在简单小鼠感染模型中测试病毒衰减

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

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在伪单体中生成帧内基因删除突变体，并在简单小鼠感染模型中测试病毒衰减

DOI:

10.3791/60297-v

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08:34 min

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January 08, 2020

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Meagan E. Valentine, Brandon D. Kirby, Hongwei D. Yu²

¹Progenesis Technologies, LLC, ²Department of Biomedical Sciences, Pediatrics,Joan C. Edwards School of Medicine at Marshall University

Chapters

00:05Title
01:24Detection of Double-crossover Recombinants of P. aeruginosa
02:24Confirmation of Gene Deletion with Colony PCR
03:07Visualization of Bioluminescence-labeled Bacteria in Mice
05:42Inoculation of Animals with Bacterial Strains
06:28Results: Evaluation of Attenuated P. aeruginosa Strain
07:24Conclusion

Summary

자동 번역

在这里，我们描述了一个简单和可重复的小鼠感染模型协议，以评估与美国食品和药物管理局（FDA）批准的大肠杆菌用于商业用途的假单抗病毒的遗传改性菌株的衰减。

在伪单体中生成帧内基因删除突变体，并在简单小鼠感染模型中测试病毒衰减

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Summary

자동 번역

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