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Cochlear Surface Preparation in the Adult Mouse
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Cochlear Surface Preparation in the Adult Mouse

Cochlear Surface Preparation in the Adult Mouse

DOI:
10.3791/60299-v

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09:51 min

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November 06, 2019

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, , ,
1Department of Pathology and Laboratory Medicine,Medical University of South Carolina, 2MOE Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences,Southeast University

Chapters

  • 00:05Title
  • 01:15Temporal Bone Extraction, Fixation, and Perfusion
  • 02:42Temporal Bone Decalcification
  • 03:31Cochlear Epithelium Microdissection
  • 05:56Cochlear Synapse Immunolabeling
  • 07:52Results: Representative Cochlear Preparation Imaging
  • 09:18Conclusion

Summary

자동 번역

자동 번역

This article presents a modified cochlear surface preparation method that requires decalcification and use of a cell and tissue adhesive to adhere the pieces of cochlear epithelia to 10 mm round cover slips for immunohistochemistry in adult mouse cochleae.

Tags

Cochlear Surface PreparationImmunohistochemistryConfocal ImagingCochlear MicrodissectionCochlear EpitheliumHair Cell RegenerationTemporal Bone ExtractionCochlear FixationEDTA Decalcification

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