Journal
/
암 연구학
/
小肠神经内分泌肿瘤类球体的建立与特征
JoVE 신문
암 연구학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 암 연구학
Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids
Please note that all translations are automatically generated. Click here for the English version.

小肠神经内分泌肿瘤类球体的建立与特征

DOI:
10.3791/60303-v

•

09:43 min

•

October 14, 2019

•

, , , , ,
1Department of Surgery,University of Iowa Carver College of Medicine, 2High Throughput Screening Facility,University of Iowa Carver College of Medicine, 3Department of Pathology,University of Iowa Carver College of Medicine

Chapters

  • 00:04Title
  • 00:44Small Bowel Neuroendocrine Tumor (SBNET) Collection and Cell Dissociation
  • 02:10Culture of SBNET as Tumor Spheroids in ECM
  • 04:02Characterization of SBNET Spheroids by Immunofluorescence
  • 05:50Splitting SBNET Spheroids
  • 07:23Results: Immunofluorescence Imaging and Drug Testing of SBNET Spheroids
  • 09:02Conclusion

Summary

자동 번역

자동 번역

神经内分泌肿瘤（NETs）来自神经峰的神经内分泌细胞。它们生长缓慢，对文化充满挑战。我们提出了一个替代策略，通过培养它们作为球体，从小肠中种植NET。这些球体有小肠NET标记，可用于药物测试。

Tags

Keywords: Small Bowel Neuroendocrine TumorSBNET3D CultureSpheroidsCell LineNeuroendocrine TumorCell IsolationCell DigestionCell CountingExtracellular Matrix

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/60303?article_type=v

在电化学疗法的操作程序在肿瘤治疗中的狗和猫
check_url/kr/60303?article_type=v

单细胞下游特征的靶细胞预富集与全基因组扩增
check_url/kr/60303?article_type=v

肺定植法在肺部组织循环肿瘤细胞可视化中的建立
check_url/kr/60303?article_type=v

来自沙柯玛患者衍生异种移植物的肿瘤启动细胞的分离和表征
check_url/kr/60303?article_type=v

高通量三维肿瘤球体药物筛选的生成
check_url/kr/60303?article_type=v

超声引导组织定向细胞植入术在生物学相关转移性肿瘤移植中的应用
check_url/kr/60303?article_type=v

三维肿瘤球体的纵向形态和生理监测
check_url/kr/60303?article_type=v

免疫荧光对非小细胞肺癌患者循环肿瘤细胞的半自动PD-L1特征化及枚举
check_url/kr/60303?article_type=v

用于肿瘤球体半高通量分析的快速光学清除
check_url/kr/60303?article_type=v

评估从3D多细胞肺肿瘤球体中分离的癌症相关成纤维细胞的线粒体健康

Read Article