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小鼠骨骼肌肉外泄原发性肌基器的分离和分化

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Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

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小鼠骨骼肌肉外泄原发性肌基器的分离和分化

DOI:

10.3791/60310-v

•

06:53 min

•

October 15, 2019

•

Megan Vaughan, Katja A. Lamia

¹Scripps Research,Department of Molecular Medicine

Chapters

00:05Title
00:46Collection and Processing of Muscle Tissue Explants
02:11Harvesting Outgrowing Myoblasts
04:16Expansion and Enrichment of Proliferating Myoblasts
05:54Results: Myoblasts and Myotubes
06:30Conclusion

Summary

자동 번역

肌细胞是增殖前体细胞，分化形成多核肌管，并最终骨骼肌肌纤维。在这里，我们提出了一个协议，从年轻的成年小鼠骨骼肌肉有效隔离和培养原发性肌体。该方法支持对培养中的肌肉细胞进行分子、遗传和代谢研究。

Tags

Keywords: Primary Myoblasts Mouse Skeletal Muscle Muscle Stem Cells Myotubes Cell Isolation Cell Culture Differentiation Metabolism Circadian Rhythms Muscle Physiology Gentamycin Plating Media Myoblast Media Trypsin Coating Solution

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