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研究使用正交乳腺癌模型的三阴性乳腺癌

JoVE 신문
암 연구학

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JoVE 신문 암 연구학

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

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研究使用正交乳腺癌模型的三阴性乳腺癌

DOI:

10.3791/60316-v

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09:29 min

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March 20, 2020

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Robert Y. S. Cheng, Nimit L. Patel, Timothy Back, Debashree Basudhar, Veena Somasundaram, Joseph D. Kalen, David A. Wink, Lisa A. Ridnour

¹Molecular Mechanism Section, Cancer and Inflammation Program, Center for Cancer Research,National Cancer Institute at Frederick, ²Small Animal Imaging Program, Frederick National Laboratory for Cancer Research,Leidos Biomedical Research, Inc.

Chapters

00:05Introduction
00:55Orthotopic Breast Cancer Model and Tumor Size Measurement
02:05In Vivo Bioluminescence and Ex Vivo Fluorescence Imaging
04:19Molecular Metastatic Breast Cancer Cell Detection
06:27Lung Metastatic Breast Cancer Cell Detection
07:29Results: Representative Bioluminescence Imaging (BLI) and GFP Signal Detection
08:52Conclusion

Summary

자동 번역

这项工作预设了一种高级协议，通过检测绿色荧光蛋白和生物发光信号以及定量分子检测技术的集成来准确评估肿瘤负荷。

Tags

Keywords: Triple Negative Breast Cancer Orthotopic Breast Cancer Model GFP Tag Bioluminescence Imaging Micrometastasis PCR Analysis Anti-cancer Agents EMT Drug Resistance Cancer Stem Cell Mechanisms Metastatic Process Anti-cancer Drug Development Xenograft Tumor Size Measurement Luciferin Kinetics Lung Metastasis Brain Metastasis

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