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A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
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A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

DOI:
10.3791/60317-v

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07:55 min

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January 07, 2020

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, ,
1Department of Chemical Engineering,Queen’s University

Chapters

  • 00:04Title
  • 00:34Cell Culture Surface Coating with Poly(Methyl Methacrylate) (PMMA)
  • 02:153T3 Cell Lysate Preparation
  • 03:26Adsorbed Protein Layer Effect on Toll-Like Receptor (TLR)-Mediated Nuclear Factor-kappa-B (NF-kappa-B) Macrophage Activity Assessment
  • 06:01Results: Representative Lysate TLR Ligand Adsorption to Different Polymer Surfaces
  • 07:18Conclusion

Summary

자동 번역

자동 번역

This protocol provides researchers with a rapid, indirect method of measuring TLR-dependent NF-кB/AP-1 transcription factor activity in a murine macrophage cell line in response to a variety of polymeric surfaces and adsorbed protein layers that model the biomaterial implant microenvironment.

Tags

Macrophage Reporter Cell AssayToll-like ReceptorNF-kBAP-1SignalingAdsorbed Protein LayersPolymeric SurfacesPMMASpin Coating3T3 Cell LysatesCell CultureCell Dissociation

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