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Journal / Immunology and Infection / 恒流中原发性人类内皮细胞的肺炎球菌感染…
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Immunology and Infection

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JoVE Journal Immunology and Infection
恒流中原发性人类内皮细胞的肺炎球菌感染
 
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恒流中原发性人类内皮细胞的肺炎球菌感染

Article DOI: 10.3791/60323-v • 09:34 min • October 31st, 2019
October 31st, 2019 •
1,2, 1,3, 1,4, 1
1Institut für Mikrobiologie, Technische Universität Braunschweig, 2Devision of Infection Medicine, Department of Clinical Science, Lund University, 3Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Ludwig Maximilians University, 4Department of Molecular Infection Biology, Helmholtz Center for Infection Research

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本研究描述了在定义的流动条件下，在剪切应力下在分化人类原发内皮细胞表面产生的对冯·威勒布兰德因子字符串的肺炎球菌依从的微观监测。通过应用微分免疫染色程序，此协议可扩展到特定细胞结构的详细可视化和细菌定量。

Tags

免疫学和感染，第152期，肺炎链球菌，微流体，内皮细胞，显微镜，荧光，剪切应力，依从性
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