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면역학 및 감염병학

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细菌细胞的三维成像，实现精确的细胞表现和精确的蛋白质定位

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

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细菌细胞的三维成像，实现精确的细胞表现和精确的蛋白质定位

DOI:

10.3791/60350-v

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06:33 min

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October 29, 2019

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Benjamin P. Bratton, Brody Barton, Randy M. Morgenstein

¹Department of Microbiology and Molecular Genetics,Oklahoma State University, ²Department of Molecular Biology and Lewis-Sigler Institute of Integrative Genomics,Princeton University

Chapters

00:04Title
00:22Slide Preparation
01:41Imaging
03:05Cell Reconstruction
05:10Results: Representative Assessment of Protein Localization to Specific Cellular Geometries
06:00Conclusion

Summary

자동 번역

该协议解释了如何准备和安装细菌样本进行活的三维成像，以及如何从这些图像中重建大肠杆菌的三维形状。

Tags

3D Imaging Bacterial Cells Cellular Representation Protein Localization Microscopy Agarose Pads E. Coli Z-stack Fluorescence Imaging

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