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Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
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Chapters
- 00:04Title
- 00:47Preparation of Iron-depleted Defined Medium
- 01:58Growing Mycobacteria in Iron-limited Conditions
- 03:44Collection, Isolation and Quantification of MEVs (Mycobacterial Extracellular Vesicles)
- 06:38Results: MEV Purification and Quantification
- 07:49Conclusion
Mycobacterium tuberculosis shows increased production and release of extracellular vesicles in response to low iron conditions. This work details a protocol for generating low iron conditions and methods for the purification and characterization of mycobacterial extracellular vesicles released in response to iron deficiency.
