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Native Polyacrylamid-Gel-Elektrophorese Immunoblot-Analyse der endogenen IRF5-Dimerisierung
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
DOI:

08:57 min

October 06, 2019

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Chapters

  • 00:04Title
  • 01:00Stimulation of CAL-1 Cells
  • 02:23Extraction of Cellular Proteins
  • 03:39Analysis of IRF5 Dimerization by Native PAGE
  • 04:55Immunoblot Analysis of IRF5
  • 06:10Results: IRF5 Dimerization
  • 07:21Conclusion

Summary

자동 번역

Eine native Western Blot-Methode zur Analyse der endogenen Interferon-Regulierungsfaktor 5-Dimerisierung in der CAL-1-Plasmazytoid-Dendritischen Zelllinie wird beschrieben. Dieses Protokoll kann auch auf andere Zelllinien angewendet werden.

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