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Chapters
- 00:04Title
- 00:47Fabrication of Polydimethylsiloxane (PDMS) Stencil and Microchannel
- 02:39Preparation of Bottom Glass with Polyacrylamide (PA) Gel
- 04:41Micropatterning of Cell Islands and Assembly of PA Gel with PDMS Microchannel
- 06:14Integrated Microfluidic System
- 07:32Image Acquisition
- 08:27Results: Mechanical Interactions of MDCK Cell Islands
- 09:55Conclusion
Collective cell migration in development, wound healing, and cancer metastasis is often guided by the gradients of growth factors or signaling molecules. Described here is an experimental system combining traction microscopy with a microfluidic system and a demonstration of how to quantify the mechanics of collective migration under biochemical gradient.
