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Chapters
- 00:05Title
- 01:12Preparation of P. aeruginosa PAO1 Culture
- 01:58Preparation of Nematode Growth Medium (NGM) Plates for Testing the Effects of Bacteria and DIM on the Intestinal Permeability of C. elegans
- 02:57Bacteria or DIM Treatment and FITC-Dextran Feeding
- 04:19Imaging of C. elegans with the Operetta Imaging System and Determination of Intestinal Permeability
- 05:43Results: Effects of Bacteria on Intestinal Permeability of P. aeruginosa
- 06:40Conclusion
Dit protocol beschrijft hoe de intestinale permeabiliteit van Caenorhabditis elegansmeten. Deze methode is nuttig voor fundamenteel biologisch onderzoek naar intestinale gezondheid in verband met de interactie tussen darmbacteriën en hun gastheer en voor screening om te identificeren van probiotische en chemische agentia te genezen van lekkende gut syndroom en inflammatoire darmziekten.
