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JoVE Journal
Biochemistry

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Label-fri Immunoprecipitation massespektrometri workflow for storstilet nuklear Interactome profilering
 
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Label-fri Immunoprecipitation massespektrometri workflow for storstilet nuklear Interactome profilering

Article DOI: 10.3791/60432-v 11:19 min November 17th, 2019
November 17th, 2019

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요약

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Beskrevet er en proteomics workflow for at identificere protein interaktion partnere fra en nuklear subcellulære fraktion ved hjælp af immun affinitet berigelse af et givet protein af interesse og label-fri massespektrometri. Arbejdsprocessen omfatter subcellulære fraktionering, immunopræcipitation, filter Aided prøveforberedelse, offline oprydning, massespektrometri, og en downstream Bioinformatik pipeline.

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Biokemi massespektrometri proteomics subcellulær fraktionering immunoprecipitation interactome bioinformatik workflow
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