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1Department of Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Science,University of Miami, 2Department of Biology,University of Miami, 3The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, and Regenerative Medicine and Stem Cell Research Center,Ben Gurion University of the Negev
Chapters
- 00:04Introduction
- 00:36Fluorescence-Activated Cell Sorting (FACS) Gating Strategy Setup and Analysis
- 01:49FACS Sorting and Cell Collection
- 02:55Results: Representative Live Coral Cell Gating
- 03:59Conclusion
Les coraux créent des écosystèmes de biodiversèses importants pour les humains et les organismes marins. Cependant, nous ne comprenons toujours pas le plein potentiel et la fonction de nombreuses cellules coralliennes. Ici, nous présentons un protocole développé pour l’isolement, l’étiquetage et la séparation des populations de cellules coralliennes pierreuses.
