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Immunology and Infection

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JoVE Journal Immunology and Infection
A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)
 

A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)

Article DOI: 10.3791/60460-v • 10:41 min • April 30th, 2020
April 30th, 2020 •
1, 1,2, 1, 1, 1, 3, 4, 1, 1
1Division of Infection and Global Health, School of Medicine, University of St Andrews, 2National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre, 3Instituto Nacional de Saúde (INS), Ministério da Saúde, 4Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences, University of Glasgow

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요약
Automatic Translation

We describe a tuberculosis molecular bacterial load assay test performed after heat inactivation of sputum. Heat inactivation renders sputum samples noninfectious and obviates the need for containment level 3 laboratories for tuberculosis molecular tests.

Tags

Tuberculosis Molecular Bacterial Load Assay TB-MBLA Disease Burden Anti-tuberculosis Medication Treatment Response Quantitative Result Tuberculosis Burden Bacterial Pathogens Nontuberculous Mycobacteria Chronic Obstructive Pulmonary Disease Cell Cultures Bacille Calmette-Guerin BCG Culture Patient Sputum Samples
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