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Chapters
- 00:00Introduction
- 02:52Embryon Harvest and Treatment
- 05:05Larvae Preparation fo Photography
- 06:05Microscopy
- 07:02Determining Integrated Density
- 08:14Representative Results
- 10:20Conclusions
Present here is a detailed protocol for the use of zebrafish embryos Tg(vtg1: mCherry) for the detection of estrogenic effects. The protocol covers the propagation of the fish and treatment of embryos, and emphasizes the detection, documentation, and the evaluation of fluorescent signals induced by endocrine disrupting compounds (EDC).
