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使用Tg（Vtg1：mcherry）斑马鱼胚胎测试内分泌干扰化合物的雌激素效应

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환경과학
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Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds

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使用Tg（Vtg1：mcherry）斑马鱼胚胎测试内分泌干扰化合物的雌激素效应

DOI:

10.3791/60462-v

•

12:02 min

•

August 08, 2020

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Zsolt Csenki, Ákos Horváth, Illés Bock, Edina Garai, Flóra Kerekes, Erna Vásárhelyi, Balázs Kovács, Béla Urbányi, Ferenc Mueller, Katalin Bakos

¹Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences,Szent István University, ²Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences,University of Birmingham

Chapters

00:00Introduction
02:52Embryon Harvest and Treatment
05:05Larvae Preparation fo Photography
06:05Microscopy
07:02Determining Integrated Density
08:14Representative Results
10:20Conclusions

Summary

자동 번역

目前这里是使用斑马鱼胚胎Tg（vtg1：mCherry）来检测雌激素效应的详细协议。该协议涵盖鱼类的繁殖和胚胎的治疗，并强调内分泌干扰化合物（EDC）引起的荧光信号的检测、文献和评估。

Tags

Keywords: Tg(Vtg1:mcherry) Zebrafish Endocrine Disrupting Compounds Estrogenic Effects Biomarker Vitellogenin In Vivo Testing Environmental Samples Animal Welfare

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